The effect of microbial challenge on the intestinal proteome of broiler chickens

O’Reilly, Emily L.; Burchmore, Richard J.; Sparks, Nicholas H.; Eckersall, P. David

doi:10.1186/s12953-017-0118-0

Proteome Science

Table 2 Spots differentially abundant for either time or between challenge and control birds. The p-values for each variable: time and litter are given for each spot, and a list of proteins identified by mass spectrometry, together with theoretical molecular weights, isoelectric points, accession number and Mascot score

From: The effect of microbial challenge on the intestinal proteome of broiler chickens

Spot	Protein	P- value Litter	P-value Time	Accession	Theoretical MW (kDa)/ Pi	Mascot score
Spots that showed significant differential abundance over time
190	Actin cytoplasmic type 5 Actin, aortic smooth muscle	0.079	0.003	gi\|56119084 gi\|71895043	42.2/5.3 42.35.23	629 559
198	Actin, cytoplasmic type 5 Actin, alpha skeletal muscle	0.083	0.003	gi\|56119084 gi\|71894831	42.1/5.3 42.3/5.23	512 471
512	Heat shock protein HSP 90-alphad Heat shock cognate protein HSP 90beta	0.113	0.006	gi\|157954047 gi\|47604960	84.4/5.01 83.7/4.95	268 112
503	Apolipoprotein A-I Alpha-tropomyosin 2	0.161	0.006	gi\|211159 27465053	30.6/5.58 32.8/4.65	589 168
194	Dermcidin preproprotein [Homo sapiens] actin, cytoplasmic type 5	0.275	0.007	gi\|16751921 gi\|56119084	11.3/6.08 42.1/5.3	108 98
106	Actin, aortic smooth muscle Actin, cytoplasmic type 5 Adenosine deaminase Alpha-enolase	0.260	0.008	gi\|71895043 gi\|56119084 gi\|57529377 gi\|46048768	42.3/5.23 42.1/5.3 41.0/5.82 47.66.17	610 539 364 113
182	Actin, cytoplasmic type 5 Actin, aortic smooth muscle	0.468	0.008	gi\|56119084 gi\|71895043	42.1/5.3 42.3/5.23	611 446
99	Hypothetical protein RCJMB04_22i13 (succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial-like Beta-actin	0.211	0.009	gi\|60099069 gi\|63018	50.3/6.92 42.0/5.29	694 287
553	Heat shock protein 70 Heat shock cognate 71 kDa protein	0.120	0.011	gi\|30962014 gi\|45384370	70.0/5.66 71.0/5.47	373 304
242	Actin-related protein 2/3 complex subunit 5	0.126	0.012	gi\|71896007	16.5/5.43	337
142	PREDICTED: protein SEC13 homolog	0.162	0.012	gi\|363738753	35.6/5.07	151
46	Desmin Vimentin	0.301	0.013	gi\|2959450 gi\|14326309	51.6/5.30 53.1/5.09	2155 209
555	78 kDa glucose-regulated protein precursor	0.068	0.013	gi\|45382769	72.0/5.12	1947
153	PREDICTED: inorganic pyrophosphatase Beta-actin Smooth muscle gamma actin	0.160	0.013	gi\|118092623 gi\|63018 gi\|2967678	33.1/5.53 42.0/5.29 42.1/5.31	526 205 198
13	Plastin-2 Actin, alpha skeletal muscle	0.300	0.017	gi\|56605886 gi\|71894831	70.2/ 5.16 42.3/5.23	936 190
130	Actin, cytoplasmic type 5 Actin, aortic smooth muscle	0.310	0.018	gi\|56119084 gi\|71895043	42.1/5.3 42.3/5.23	879 816
138	PREDICTED: pyridoxal kinase	0.376	0.023	gi\|363728772	35.1/5.93	387
128	Actin, cytoplasmic type 5 Actin, aortic smooth muscle	0.300	0.031	gi\|56119084 gi\|71895043	42.1/5.3 42.3/5.23	806 744
557	Tubulin beta-7 chain Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-2 chain	0.359	0.036	gi\|45384338 gi\|153792017 gi\|71896203 gi\|52138699	50.0/4.78 50.2/4.78 50.8/4.86 50.3/4.78	614 470 416 399
166	Coatomer subunit delta Annexin A6	0.370	0.042	gi\|118405190 gi\|45382029	57.5/5.8 75.6/5.42	281 163
58	Alpha-enolase Hypothetical protein RCJMB04_24e12	0.440	0.042	gi\|46048768 gi\|53135040	47.6/6.17 23.8/6.97	490 305
146	Beta-actin Smooth muscle gamma actin	0.068	0.042	gi\|63018 gi\|2967678	42.0/5.29 42.2/5.31	230 174
Spots that showed significant differential abundance between challenged and control birds
525	Villin-1	0.042	0.050	gi\|45382125	92.8/5.88	87
526	Villin-1	0.046	0.065	gi\|45382125	92.8/5.88	513
135	Actin, cytoplasmic type 5 PREDICTED: actin, alpha skeletal muscle B isoform 2	0.049	0.118	gi\|56119084 gi\|50803534	42.1/5.3 42.3/5.16	364 300
17	T-complex protein 1 subunit eta	0.048	0.239	gi\|71895883	60.8/5.96	716
157	Actin, aortic smooth muscle Proliferating cell nuclear antigen	0.046	0.330	gi\|71895043 gi\|45383776	42.3/5.23 29.1/4.6	618 593
104	Adenosine deaminase Actin, aortic smooth muscle	0.042	0.358	gi\|57529377 gi\|71895043	41.0/5.82 42.3/5.23	620 329

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