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Table 2 Phosphoproteins and phosphosites in zebra finch eggshell matrix

From: The calcified eggshell matrix proteome of a songbird, the zebra finch (Taeniopygia guttata)

Accession (protein)

Peptide

Number of P-sites

Proba-bility 1

Mod/unmod

Gi|224045100 Ovalbumin-like

57AIHFDKIPGFGEpSVESQCGASVSIHNSLK86

1

1

221/5

 

63IPGFGEpSVESQCGASVSIHNSLK86

1

1

748/5

 

342VAGp[ S S]GAGVDDTSVSEEIR360

1

[1]

37/1333

Gi|224049274/H0YVI0 Ovocleidin-116

94REPPAGSAGTAPEHSDNp[ S ST]EVVEYGIVFKPK125

1

[1]

123/124

 

228GAGDEGpSGEATVSGQGQEGVK248

1

1

445/169

 

249QGTGTGGVALp[S S ]VTEK265

1

[>0.9]

51/681

 

355RLDVTAAPpSGEDDSIPTHR373

1

0.99

3/40

 

387GDSVApS SLR395

1

0.85

1/59

 

396DGHLpTGEDEEGATTIGVDR414

1

1

7/543

 

508GGpSGEVGTTTSRPSR522

1

1

9/3

 

546VDTAPSPSGKPSGWASSGAQTSAGGHGDAGRp[SHAS ]K582

1

[1]

21/0

 

556PSGWASSGAQTSAGGHGDAGRp[SHAS]K582

1

[1]

15/15

Gi|224057610/H0Z847 Vitellogenin-2

1056IINEVNAEpSEEEGESSLYGDMK1077

1

1

3/0

 

1550MPApSELQPPIWNVFAAPSAVLENLK1575

1

1

34/1

Gi|224057612 Vitellogenin-2-like

1218AMpSQPEFLGDSKPPILAAVLR1238

1

1

1/21

H0Z8U7/gi|224057648 Similar to vitellogenin-1

1252 TApSFPLASAAEGER1265

1

1

35/17

Gi|449474881 Similar to ovoinhibitor

602 pSGEAIAACPYILR614

1

1

35/10

H0Z5S2/gi|449479753 Similar to PEDF

291EpTRLQSLFTSPDFSK305

1

1

1/0

Gi|449480130/H0Z5Q3 Similar to alpha-2-antiplasmin

2374EAQDpSREATDANEYRVPK2391

1

1

1/0

  1. Phosphorylation sites are presented in bold print. In some cases it was not possible to unequivocally identify the exact phosphorylation site. One cause may be partial phosphorylation of neighboring target residues as shown in square brackets. Amino acid residues with the higher prediction score [74] are underlined in such instances. Underlined amino acids outside square brackets indicate alternative minor phosphorylation sites with a probability lower than 0.75. 1, only the highest location probability (MaxQuant) is indicated