Spot # | Protein namea | NCBI Accession # | Protein MW | Protein PI | Pep.Count | Protein score | Protein Score C. I. % | Total ion score | Total Ion C. I. % | Expression difference HY versus LY (WAP)b | Temporal expression trend in HY (WAP)b | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 |  |  |  |  |  |  |  |  |  | DIGE | Western analysis | DIGE | Western analysis |
 | Lipid metabolism |  |  |  |  |  |  |  |  |  |  |  |  |
63 | phospholipase D [Oryza sativa] | gi|1020415 | 92151 | 5.6 | 11 | 130 | 100.0 | 40 | 67.4 | down (16) | down (16) | down (16) | up (16, 18) |
67 | biotin carboxylase subunit [Glycine max] | gi|3219361 | 58770 | 7.2 | 11 | 302 | 100.0 | 222 | 100.0 | - | - | up (16, 18) | up (16, 18) |
 | Carbohydrate metabolism |  |  |  |  |  |  |  |  |  |  |  |  |
26 | glyceraldehyde 3-phosphate dehydrogenase [Elaeis guineensis] | gi|82400215 | 32136 | 7.1 | 7 | 191 | 100.0 | 122 | 100.0 | down (16) | down (12, 16) | - | up (18) |
74 | fructose-biphosphate aldolase [Elaeis guineensis] | gi|192910908 | 38578 | 6.7 | 8 | 174 | 100.0 | 119 | 100.0 | up (16) | up (16) | up (16, 18) | up (16, 18) |
37 | Triosephosphate isomerase, chloroplastic [Secale cereale] | gi|1174745 | 31613 | 6.0 | 7 | 103 | 100.0 | 56 | 99.4 | down (16) | down (12, 16) | - | up (18) |
 | Energy metabolism |  |  |  |  |  |  |  |  |  |  |  |  |
44 | NADPH:quinone reductase family protein [Arabidopsis thaliana] | gi|30687535 | 21778 | 6.1 | 4 | 185 | 100.0 | 155 | 100.0 | down (12) | nt | up (16) | nt |
46 | FQR1 (flavodoxin-like quinone reductase 1) [Arabidopsis thaliana] | gi|15239652 | 21782 | 6.0 | 4 | 130 | 100.0 | 100 | 100.0 | down (12) | nt | up (16) | nt |
75 | short chain type dehydrogenase [Ricinus communis] | gi|223550344 | 33332 | 9.5 | 3 | 104 | 100.0 | 88 | 100.0 | - | nt | up (16, 18) | nt |
51 | mitochondrial peroxiredoxin [Pisum sativum] | gi|47775654 | 21463 | 8.4 | 3 | 140 | 100.0 | 120 | 100.0 | up (16) | down (16) | up (16, 18) | up (18) |
 | Amino acid metabolism |  |  |  |  |  |  |  |  |  |  |  |  |
21 | o-methyltransferase [Ricinus communis] | gi|223540510 | 40000 | 5.3 | 4 | 92 | 100.0 | 68 | 99.9 | down (16) | down (16) | down (16, 18) | up (18) |
5 | methionine synthase protein [Sorghum bicolor] | gi|18483235 | 83736 | 5.9 | 12 | 211 | 100.0 | 166 | 100.0 | down (16) | - | - | up (16, 18) |
65 | 5-methyltetrahydropteroyltri-glutamate-homocysteine methyltransferase [Oryza sativa] | gi|108862992 | 84582 | 5.9 | 9 | 84 | 99.7 | 39 | 71.7 | - | - | down (16, 18) | up (18) |
30 | VFB2 (VIER F-BOX PROTEINE 2); ubiquitin-protein ligase [Arabidopsis thaliana] | gi|18409012 | 57212 | 8.7 | 8 | 65 | 80.5 | - | - | up (18) | down (18) | - | up (18) |
24 | proline iminopeptidase, putative [Ricinus communis] | gi|223538638 | 44547 | 6.0 | 5 | 94 | 100.0 | 71 | 100.0 | up (18) | up (18) | up (16, 18) | up (16, 18) |
 | Stress related proteins |  |  |  |  |  |  |  |  |  |  |  |  |
80 | 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1–156) [Arabidopsis thaliana] | gi|15220832 | 17593.0 | 5.4 | 5 | 70 | 94.0 | 30 | - | - | - | up (16. 18) | up (18) |
7 | OSJNBb0085F13.17 [Oryza sativa] | gi|38345312 | 80199.8 | 5.1 | 12 | 102 | 100.0 | 28 | - | down (16) | nt | - | nt |
54 | glutathione peroxidase [Triticum monococcum] | gi|148529480 | 11668.8 | 5.7 | 3 | 73 | 97.0 | 50 | 97.9 | up (16) | down (16) | - | up (18) |
41 | glutathione S-transferase Phi [Matricaria chamomilla] | gi|17385642 | 25264.9 | 6.0 | 5 | 96 | 100.0 | 69 | 100.0 | down (16) | - | up (16) | up (16, 18) |
43 | glutathione-S-transferase theta, gst, putative [Ricinus communis] | gi|223528475 | 24482.6 | 5.8 | 1 | 82 | 99.6 | 77 | 100.0 | up (18) | nt | up (16, 18) | nt |
78 | abscisic stress ripening protein [Musa acuminata] | gi|219963178 | 16735.8 | 5.6 | 3 | 76 | 98.5 | 51 | 97.8 | - | nt | down (16, 18) | nt |
53 | temperature-induced lipocalin [Elaeis guineensis] | gi|192911934 | 21614.8 | 5.7 | 5 | 93 | 100.0 | 51 | 98.8 | up (16) | - | up (16) | up (18) |
 | Structure related proteins |  |  |  |  |  |  |  |  |  |  |  |  |
22 | actin [Populus trichocarpa] | gi|224117708 | 40592.4 | 5.1 | 15 | 375 | 100.0 | 258 | 100.0 | down (16) | - | - | up (18) |
23 | caffeic acid O-methyltransferase [Vanilla planifolia] | gi|45444737 | 40632.5 | 5.7 | 4 | 92 | 100.0 | 72 | 100.0 | down (12, 16) | down (12, 16) | down (16, 18) | - |
73 | fibrillin-like protein [Elaeis guineensis] | gi|32250939 | 18991.0 | 5.0 | 7 | 109 | 100.0 | 37 | 46.0 | up (12) | nt | down (16) | nt |
68 | Os12g0163700 (actine gamma 2) [Oryza sativa] | gi|115487492 | 39837.0 | 5.3 | 10 | 137 | 100.0 | 62 | 99.9 | - | - | up (16, 18) | up (18) |
 | Other Proteins |  |  |  |  |  |  |  |  |  |  |  |  |
13 | V-type proton ATPase catalytic subunit A [Daucus carota] | gi|137460 | 68792 | 5.3 | 17 | 250 | 100.0 | 107 | 100.0 | down (16) | down (16) | down (16) | up (18) |
9 | catalase 2 [Elaeis guineensis] | gi|192910916 | 56922.4 | 6.5 | 11 | 100 | 100.0 | 25 | - | up (18) | up (18) | - | up (18) |
56 | large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase [Chlorogonium elongatum] | gi|74267421 | 41910.0 | 7.0 | 5 | 71 | 95.3 | 40 | 73.0 | up (18) | up (18) | up (16, 18) | up (18) |
32 | Ran GTPase binding protein [Ricinus communis] | gi|223434074 | 119713.4 | 9.0 | 12 | 67 | 87.9 | - | - | up (12) | - | up (16, 18) | up (18) |
72 | ribosomal protein L10 [Elaeis guineensis] | gi|192910684 | 34609.4 | 5.4 | 6 | 251 | 100.0 | 198 | 100.0 | down (12) | down (12) | up (16, 18) | up (16, 18) |
49 | ABC1 family protein [Arabidopsis thaliana] | gi|79325958 | 73790.7 | 5.6 | 13 | 73 | 96.7 | - | - | down (18) | - | down (18) | up (16, 18) |
48 | Lea1P [Daucus carota] | gi|10945667 | 16361.9 | 7.8 | 7 | 66 | 84.5 | - | - | up (16) | - | up (16) | up (18) |
33 | nascent polypeptide associated complex alpha [Vitis vinifera] | gi|225470846 | 22013.8 | 4.3 | 5 | 305 | 100.0 | 262 | 100.0 | up (12) | down (12) | down (16, 18) | up (16, 18) |
58 | Os02g0753300 [Oryza sativa] | gi|115448731 | 19972.8 | 6.2 | 2 | 87 | 99.9 | 71 | 100.0 | up (16) | nt | - | nt |
66 | Os05g0482700 [Oryza sativa] | gi|115464537 | 60774.8 | 5.3 | 10 | 256 | 100.0 | 183 | 100.0 | - | nt | down (16) | nt |
84 | regulator of ribonuclease activity A [Zea mays] | gi|195638112 | 18105.2 | 5.8 | 5 | 80 | 99.4 | 34 | - | - | nt | up (16, 18) | nt |
36 | retroelement pol polyprotein-like [Arabidopsis thaliana] | gi|9759493 | 126284.1 | 9.0 | 15 | 66 | 85.2 | - | - | down (12) | nt | up (16) | nt |
59 | conserved hypothetical protein [Ricinus communis] | gi|223539131 | 16333.4 | 6.6 | 1 | 55 | - | 49 | 97.5 | up (16) | nt | up (16, 18) | nt |
34 | predicted protein [Ostreococcus lucimarinus CCE9901] | gi|145345862 | 14575.0 | 4.6 | 3 | 66 | 85.2 | 46 | 92.4 | up (16) | nt | up (16) | nt |
79 | predicted protein [Physcomitrella patens] | gi|168027637 | 23578.3 | 6.9 | 2 | 70 | 93.8 | 58 | 99.7 | - | nt | up (16) | nt |
55 | hypothetical protein isoform 1 [Vitis vinifera] | gi|225434277 | 22608.8 | 8.7 | 5 | 88 | 99.9 | 54 | 99.0 | up (12) | nt | down (18) | nt |