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Table 8 Identification and quantification of farinins, purinins, triticins, globulins, and grain softness proteins by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot #

Predominant protein1

Total

proteins

in spot2

Spot

Volume3

Std

Dev4

MS/MS

Coverage5,8

Predicted

MW6,8

pI7,8

Farinins

      

196

Farinin Bu-1 full length

1

0.04

0.007

15

29978

8.1

385

Farinin Bu-1 C-terminus

1

0.07

0.013

13

18832

8.4

386

Farinin Bu-1 C-terminus

1

0.14

0.030

24

18832

8.4

193

Farinin Bu-2

2

0.28

0.052

38

30567

7.5

207

Farinin Bu-2

1

0.04

0.037

25

30567

7.5

549

Farinin Bu-2

1

<0.01

0.000

20

30567

7.5

336

Farinin Bu-3

2

0.33

0.043

45

30883

7.9

345

Farinin Bu-3

3

<0.01

0.000

34

30883

7.9

Purinins

      

542

Purinin Bu-1

3

0.07

0.007

24

20272

5.9

543

Purinin Bu-1

2

0.22

0.015

38

20272

5.9

219

Purinin Bu-2

1

0.06

0.015

34

20592

6.2

223

Purinin Bu-2

1

0.20

0.030

34

20592

6.2

220

Purinin Bu-3

1

0.23

0.018

19

22371

6.2

227

Purinin Bu-3

1

0.04

0.005

28

22371

6.2

Triticins (Tri-1)

      

143

Triticin [GenBank:DR736644] N-terminal subunit

3

0.27

0.011

inc

inc

inc

136

Triticin TC11_285558, N-terminal subunit

2

0.17

0.011

36

40501

6.2

348

Triticin TC11_285558, N-terminal subunit

2

0.23

0.023

36

40501

6.2

423

Triticin TC11_285558, N-terminal subunit

1

0.46

0.059

36

40501

6.2

463

Triticin TC11_285558, N-terminal subunit

1

0.06

0.003

18

40501

6.2

249

Triticin TC11_264477, C-terminal subunit

1

0.21

0.011

22

21830

8.1

253

Triticin TC11_264477, C-terminal subunit

1

0.19

0.010

17

21830

8.1

Globulins

      

218

Globulin-1 [GenBank:ABG68030] (Glo-2)

1

0.05

0.023

48

22941

8.6

104

Globulin-2 Bu-17295

1

0.02

0.004

25

53832

6.6

121

Globulin-2 Bu-17295

1

0.04

0.002

23

53832

6.6

99

Globulin-2 Bu-17366

1

0.03

0.002

inc

inc

7.0

103

Globulin-2 Bu-18428

1

0.06

0.003

10

53554

6.6

106

Globulin-2 Bu-18428

1

0.03

0.004

30

53554

6.6

180

Globulin Glo-3-type TC234094

5

0.08

0.007

inc

inc

inc

184

Globulin Glo-3-type TC11_305389

2

0.03

0.008

inc

inc

inc

309

Globulin Glo-3-type TC11_ 305389

1

0.02

0.004

inc

inc

inc

2729

Globulin Glo-3-type TC234094/WTAI-CM3 [SwissProt: P17314]

2

0.02

0.003

nd

nd

nd

GSP and Puroindoline (Pin-D1)

      

174

Grain softness protein [GenBank:CAA56591]

1

0.02

.001

17

16157

8.1

275

Grain softness protein [GenBank:CAA56586]

2

0.06

0.009

30

16381

7.6

248

Puroindoline-b [GenBank:AAT40244]

2

0.21

0.053

28

14812

9.0

271

Puroindoline-b [GenBank:AAT40244]

1

0.04

0.015

44

14812

9.0

  1. Loci are in parentheses.
  2. 1-7 Footnotes as in Table 4.
  3. 8inc indicates sequence was incomplete. nd indicates not determined because no single protein had a predominant number of peptides.
  4. 9Spot contained a similar number of peptides from two different proteins. Details are in Additional file 1.