Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Dupont, Frances M; Vensel, William H; Tanaka, Charlene K; Hurkman, William J; Altenbach, Susan B

doi:10.1186/1477-5956-9-10

Proteome Science

Table 3 Identification and quantification of HMW-GS by 2DE-MS/MS.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

Spot #	Predominant protein¹	Total proteins²	Spot Volume³	Std Dev⁴	MS/MS Coverage⁵	Predicted MW⁶	pI⁷
	HMW-GS Ax2* (Glu-A1b-1)
12	HMW-GS Ax2* [GenBank:AAB02788]	1	1.22	0.024	52	86336	5.7
16	HMW-GS Ax2* [GenBank:AAB02788]	2	0.07	0.004	13	86336	5.7
17	HMW-GS Ax2* [GenBank:AAB02788]	1	0.36	0.019	34	86336	5.7
18	HMW-GS Ax2* [GenBank:AAB02788]	1	0.05	0.001	7	86336	5.7
19	HMW-GS Ax2* [GenBank:AAB02788]	1	0.14	0.005	10	86336	5.7
23	HMW-GS Ax2* [GenBank:AAB02788]	1	0.40	0.006	26	86336	5.7
24	HMW-GS Ax2* [GenBank:AAB02788]	1	0.13	0.006	22	86336	5.7
28	HMW-GS Ax2* [GenBank:AAB02788]	1	0.03	0.004	8	86336	5.7
		Total	2.40
	HMW-GS Bx7 (Glu-B1c-1)
20	HMW-GS Bx7 [GenBank:CAA32115]	1	1.79	0.053	56	82527	8.4
21	HMW-GS Bx7 [GenBank:CAA32115]	1	2.28	0.063	57	82527	8.4
25	HMW-GS Bx7 [GenBank:CAA32115]	1	0.12	0.026	6	82527	8.4
26	HMW-GS Bx7 [GenBank:CAA32115]	1	0.48	0.016	10	82527	8.4
27	HMW-GS Bx7 [GenBank:CAA32115]	1	0.18	0.004	5	82527	8.4
29	HMW-GS Bx7 [GenBank:CAA32115]	1	0.02	0.002	3	82527	8.4
33	HMW-GS Bx7 [GenBank:CAA32115]	1	0.05	0.002	3	82527	8.4
302	HMW-GS Bx7 [GenBank:CAA32115]	1	0.10	0.009	3	82527	8.4
		Total	5.02
	HMW-GS By9 (Glu-B1c-2)
40	HMW-GS By9 [GenBank:CAA43361]	2	0.38	0.003	34	73518	8.4
47	HMW-GS By9 [GenBank:CAA43361]	1	0.05	0.002	10	73518	8.4
52	HMW-GS By9 [GenBank:CAA43361]	1	0.05	0.004	19	73518	8.4
349	HMW-GS By9 [GenBank:CAA43361]	1	0.11	0.016	27	73518	8.4
350	HMW-GS By9 [GenBank:CAA43361]	1	1.32	0.004	56	73518	8.4
351	HMW-GS By9 [GenBank:CAA43361]	2	0.15	0.017	45	73518	8.4
358	HMW-GS By9 [GenBank:CAA43361]	1	0.08	0.007	20	73518	8.4
360	HMW-GS By9 [GenBank:CAA43361]	1	0.14	0.021	48	73518	8.4
		Total	2.28
	HMW-GS Dx5 (Glu-D1d-1)
3	HMW-GS Dx5 [GenBank:ABG68042]	1	2.37	0.020	50	87941	6.1
5	HMW-GS Dx5 [GenBank:ABG68042]	1	0.70	0.043	45	87941	6.1
7	HMW-GS Dx5 [GenBank:ABG68042]	1	0.16	0.007	3	87941	6.1
10	HMW-GS Dx5 [GenBank:ABG68042]	1	0.06	0.004	19	87941	6.1
14	HMW-GS Dx5 [GenBank:ABG68042]	1	0.05	0.007	16	87941	6.1
36	HMW-GS Dx5 [GenBank:ABG68042]	1	0.07	0.006	4	87941	6.1
138	HMW-GS Dx5 [GenBank:ABG68042]	1	0.21	0.024	4	87941	6.1
307	HMW-GS Dx5 [GenBank:ABG68042]	1	0.08	0.028	21	87941	6.1
538	HMW-GS Dx5 [GenBank:ABG68042]	1	0.05	0.002	3	87941	6.1
		Total	3.75
	HMW-GS Dy10 (Glu-D1d-2)
42	HMWGS Dy10 [Swiss-Prot:P10387]	2	2.32	0.051	57	67475	7.0
48	HMWGS Dy10 [Swiss-Prot:P10387]	1	0.21	0.032	57	67475	7.0
51	HMWGS Dy10 [Swiss-Prot:P10387]	2	0.17	0.014	57	67475	7.0
333	HMWGS Dy10 [Swiss-Prot:P10387]	1	0.65	0.030	57	67475	7.0
407	HMWGS Dy10 [Swiss-Prot:P10387]	1	0.07	0.019	57	67475	7.0
424	HMWGS Dy10 [Swiss-Prot:P10387]	1	0.20	0.014	57	67475	7.0
514	HMWGS Dy10 [Swiss-Prot:P10387]	1	0.07	0.010	57	67475	7.0
		Total	3.69

Loci are indicated within parentheses.
¹Protein that matched the greatest number of peptides detected in spot by MS/MS.
²Identifications of all proteins within each spot are given in Additional file 1.
³Normalized spot volume, average of three determinations.
⁴Standard deviation for three determinations.
⁵Percentage coverage of the mature protein by identified peptides.
⁶Predicted size of the mature protein in Daltons detemined by ExPASy ProtParam.
⁷Predicted pI for the mature protein determined by ExPASy ProtParam.

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ISSN: 1477-5956

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