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Table 2 2DE-MS/MS identification of wheat flour proteins.

From: Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry

  

Scaffold Assignments1

Final Assignments2

Protein type

Total

Spots 3

Total

Sequences

NCBI

nr

Contig

databases 4

Butte 86

Contigs

or ESTs 5

Final

Sequences

Butte 86

Contigs or

ESTs 5

HMW-GS

43

6

4

2

0

5

0

LMW-GS

35

24

9

4

11

22

10

Gamma-gliadins

34

13

2

1

10

13

10

Omega-gliadins

13

8

2

2

4

7

4

Alpha-gliadins

33

34

8

16

10

23

16

Farinins

11

3

0

0

3

3

3

Purinins

6

3

0

0

3

3

3

Triticins

9

3

0

3

0

3

0

Globulins

11

8

1

4

3

8

3

GSPs/Puroindolines

4

3

3

0

0

3

0

Alpha-amylase inhibitors

21

16

9

7

0

16

0

Serpins

15

7

2

5

0

9

6

Beta-amylases

12

4

0

1

3

4

3

Other enzymes6

42

30

12

18

0

33

0

Other proteins

15

6

3

2

1

5

1

Total

 

168

55

65

48

157

59

  1. Numbers of spots and protein sequences and their database origins for each major protein category.
  2. 1Scaffold assignments for each spot are in Additional files 4, 5, 6, 7, 8, 9.
  3. 2Final assignments for each spot are in Additional file 1.
  4. 3Includes all 2-DE spots in which two or more peptides corresponded to sequences for the indicated protein type.
  5. 4Includes TaGI Releases 10.0 and 11.0, US Wheat Genome Project Assembly, HarvEST 1.14 and NCBI Unigene Build #55.
  6. 5Sequences derived from Butte 86 contigs are in Additional files 2, 3. Butte 86 sequences for some protein types were not analyzed in this study.
  7. 6Includes sequences for nine enzymes that were minor components of other spots as detailed in Additional file 1.
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Proteome Science

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