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Table 1 Comparison of MS/MS identifications of proteins in 2-DE spots from a total 2% SDS protein extract with those from UPP and EPP fractions

From: Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry

 

Total 2% SDS extract1

UPP peak 1

UPP peak 2

EPP peak 1

EPP peak 2

Spot #2

Predominant protein3

Predominant protein4

Predominant protein4

Predominant protein4

Predominant protein4

51

HMW-GS Dy10

HMW-GS By9

   

124

Alpha gliadin Bu-BQ807130

LMW-GS Bu-3 (s-type)

  

Alpha gliadin TC11_300663

125

LMW-GS [GenBank: AAB48469] (i-type)

LMW-GS Bu-3 (s-type)

   

134

Gamma gliadin Bu-5

Triticin

Gamma gliadin Bu-5

 

Gamma gliadin Bu-5

161

LMW-GS Bu-3 (s-type)

   

Alpha gliadin Bu-27

163

Gamma gliadin Bu-11

LMW-GS Bu-8 (m-type)

Alpha gliadin Bu-15

Alpha gliadin TC11_2842705

Alpha gliadin Bu-15

    

Glyceraldehyde phosphate dehydrogenase

 

166

Gamma gliadin Bu-4

LMW-GS Bu-1 (m-type)

Gamma gliadin Bu-4

Gamma gliadin Bu-4

Gamma gliadin Bu-4

169

Gamma gliadin Bu-4

LMW-GS Bu-1 (m-type)

LMW-GS Bu-1 (m-type)

Gamma gliadin Bu-4

Gamma gliadin Bu-3

172

Gamma gliadin Bu-10 or Bu-3

Gamma gliadin Bu-3

Alpha gliadin Bu-9

 

Alpha gliadin Bu-12

 

Alpha gliadin Bu-1 or Bu-3

    
 

Alpha gliadin Bu-9

    

177

Alpha gliadin Bu-BQ806209

   

Alpha gliadin TC11_315573

310

LMW-GS Bu-3 (s-type)

LMW-GS Bu-20 (s-type)

   

315

LMW-GS Bu-11 (m-type)

LMW-GS Bu-2 (s-type)

   

316

LMW-GS Bu-3 (s-type)

LMW-GS Bu-2 (s-type)

   

318

LMW-GS Bu-2/1-2/-13 (s-type)

   

LMW-GS Bu-11 (m-type)

319

LMW-GS TC11_277270 (m-type)

LMW-GS Bu-12 (s-type)

   

320

Gamma gliadin Bu-5

LMW-GS Bu-2 (s-type)

Gamma gliadin Bu-5

 

Gamma gliadin Bu-5

  

Gamma gliadin Bu-5

   

323

Gamma gliadin Bu-5

LMW-GS Bu-2 (s-type)

Gamma gliadin Bu-5

 

Gamma gliadin Bu-5

324

Gamma gliadin Bu-5

LMW-GS Bu-2 (s-type)

Gamma gliadin Bu-5

 

Gamma gliadin Bu-5

325

Gamma gliadin Bu-1 or Bu-8

Gamma gliadin Bu-2

Gamma gliadin Bu-1

 

Gamma gliadin Bu-1

  

LMW-GS Bu-7 (m-type)

   

327

Alpha gliadin Bu-11

Alpha gliadin TC11_314676 (like alpha Bu-12)

Alpha gliadin Bu-11

 

Alpha gliadin Bu-11

328

Alpha gliadin Bu-12

 

Gamma hordein

 

Gamma hordein

329

Alpha gliadin Bu-12

 

Alpha gliadin Bu-12

Glyceraldehyde phosphate dehydrogenase

Alpha gliadin Bu-12

330

Alpha gliadin Bu-2

LMW-GS Bu-8 (m-type)

Alpha gliadin Bu-15

Alpha gliadin TC11_2842705

Alpha gliadin Bu-12

331

Alpha gliadin Bu-14

 

Alpha gliadin Bu-4

 

Alpha gliadin Bu-4

 

Alpha gliadin Bu-12

    

334

Alpha gliadin Bu-BQ838853

 

Alpha gliadin Bu-2

Farinin Bu-3

Gamma gliadin Bu-7

 

Gamma gliadin-Bu-7

   

Alpha gliadin Bu-5 or Bu-14

341

Alpha gliadin Bu-23

Alpha gliadin Bu-23

Alpha gliadin Bu-23

Glyceraldehyde phosphate dehydrogenase

Alpha gliadin Bu-8

342

Alpha gliadin Bu-1

 

Alpha gliadin Bu-1

Gamma gliadin Bu-3

Alpha gliadin Bu-1

343

LMW-GS Bu-8 (m-type)

LMW-GS Bu-1 (m-type)

   
  

Gamma gliadin Bu-3

   

346

Gamma gliadin Bu-6

Alpha gliadin Bu-2

Gamma gliadin Bu-6

Aspartic proteinase

Gamma gliadin Bu-6

348

Triticin

LMW-GS Bu-7 (m-type)

   

424

HMW-GS Dy10

HMW-GS By9

   

467

Alpha gliadin Bu-4

Alpha gliadin Bu-4

Alpha gliadin Bu-4

LMW-GS Bu-8 (m-type)

Alpha gliadin Bu-4

  

LMW-GS Bu-8 (m-type)

   

468

Alpha gliadin Bu-3

Alpha gliadin Bu-3

Alpha gliadin Bu-3

Glyceraldehyde phosphate dehydrogenase

Alpha gliadin Bu-3

  

LMW-GS Bu-8 (m-type)

   

527

Gamma gliadin Bu-11

 

Alpha gliadin Bu-12

 

Gamma gliadin Bu-11

546

Alpha gliadin Bu-14

Alpha gliadin Bu-2

   

550

Alpha gliadin Bu-10

Alpha gliadin Bu-12

 

Gamma gliadin Bu-4

Alpha gliadin Bu-12

  1. 1Data is from Dupont et al.,[2].
  2. 2Spot numbers correspond to those in Dupont et al.,[2].
  3. 3Determined in Dupont et al.,[2] on the basis of the number of unique peptides assigned to each protein sequence. Where similar numbers of unique peptides were assigned to more than one protein, multiple identifications are reported.
  4. 4Based on the number of spectra that were associated with each protein sequence. For each spot the protein with the greatest number of spectra was deemed to be the predominant protein. Where similar numbers of spectra were associated with multiple proteins, more than one identification is reported.
  5. Data is shown only for spots that yielded different proteins in the different fractions.
  6. 5Contains 7 cysteines.